NISC - NIH Intramural Sequencing Center Services

Services

We aim to provide the highest quality sequencing data possible to the NIH community. We meet with every investigator interested in starting a sequencing project to discuss the best sequencing approach for their research needs. Please note that our sequencing service is restricted to intramural investigators at the NIH.

Common Sequencing Projects



Example Sources of RNA or DNA

        

Initiating A Project

human, mouse, rat, black bear, dog, chicken, bat, honeycreeper, zebrafinch, butterfly, horsefly, fly, mosquito, tick, squid, jellyfish, Mnemiopsis , Hydractinia, zebrafish, Caenorhabditis elegans, Schizosaccharomyces pombe, fungus, Plasmodium
Non-human primates: chimpanzee, gibbon, Rhesus macaque
Bacteria: Acinetobacter, Aeromonas, Citrobacter, Clostridium, Corynebacterium, E.coli, Enterobacter, Enterococcus, Helicobacter, Klebsiella, Moraxella, Pantoae, Staphylococcus
  If you are thinking about initiating a new sequencing project, please visit http://www.nisc.nih.gov/contact.cgi. After this form is submitted, we will set up a meeting with you to plan the best approach for your needs. Please note that our sequencing service is restricted to intramural investigators at the NIH. Human DNA samples will need to be properly consented and de-identified.
 

Bioinformatics

        

Library Construction

The raw data from the sequencers is extensively processed and data is evaluated for quality. The general output is BAM files for Illumina sequence data that contain information about sequence quality and alignment to a reference in addition to the sequence. For some applications, additional bioinformatics support is available (see FAQs).   NISC has a highly skilled library construction group able to make a wide range of libraries compatible with all of our sequencing platforms. You deliver high-quality DNA or RNA and we construct the library. We prefer to start a sequencing project at this point, which allows our experienced hands to control each step for optimal performance and ensure the highest rate of success at the end.