Services
We aim to provide the highest quality sequencing data possible to the NIH community. We meet with every investigator interested in starting a sequencing project to discuss the best sequencing approach for their research needs. Please note that our sequencing service is restricted to intramural investigators at the NIH.
Common Sequencing Projects
Whole genome sequencing (Eukaryotic and microbial) - FAQ
Bulk RNA-Seq - FAQ
Single-Cell RNA-Seq - FAQ
ChIP-Seq - FAQ
Amplicon sequencing
Custom capture projects - FAQ
Whole Exome Sequencing and Analysis - FAQ
Initiating A Project |
Library Construction |
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| If you are thinking about initiating a new sequencing project, please visit https://www.nisc.nih.gov/contact.cgi. After this form is submitted, we will set up a meeting with you to plan the best approach for your needs. Please note that our sequencing service is restricted to intramural investigators at the NIH. Human DNA samples will need to be properly consented and de-identified. | NISC has a highly skilled library construction group able to make a wide range of libraries compatible with all of our sequencing platforms. You deliver high-quality DNA or RNA and we construct the library. We prefer to start a sequencing project at this point, which allows our experienced hands to control each step for optimal performance and ensure the highest rate of success at the end. | |
Data Return |
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| The raw data from the sequencers is extensively processed, and data is evaluated for quality. For Illumina data, the general deliverable is fastq files which contain the base calls as well as sequence quality scores. | ||
